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Schizosaccharomyces pombe Postgenome Database

Gene name SPCC622.15c
Gene ID 35/A10
Gene synonyms/obsolete
Gene product hypothetical protein; sequence orphan
Entry clone Cloned
ORF length (unspliced) 1674
ORF length (spliced)
Entry clone length 1674
No. of intron 0
Sequence status Finished
Sequence results 924T:C / 952C:T
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPCC622.15.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGAATGAAGATGAAACTTC
Rev primer name SPCC622.15.Rv
Rev primer SEQ AGAAAGCTGGGTAAATATGGTCATAAGGCGCA
Amino acid length 557
Molecular weight 61.8
Isoelectric point (calc.) 6.6
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytoplasmic dots; nuclear dots
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 3 images) See all images

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