Gene name |
SPAC2F3.09 |
Gene ID |
35/B01 |
Gene synonyms/obsolete |
|
Gene product |
5-aminolevulinate
synthase activity; involved in glycine metabolism and heme
biosynthesis |
Entry clone |
Cloned |
ORF length (unspliced) |
1677 |
ORF length (spliced) |
|
Entry clone length |
1677 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
349T:C / 788T:C |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC2F3.09.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGAACGCGTTGTGAAATT |
Rev primer name |
SPAC2F3.09.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAATGAGTCGTAGTAAAAGGA |
Amino acid length |
558 |
Molecular weight |
60.9 |
Isoelectric point (calc.) |
9.1 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LEAKLASLPL |
Localization (YFP) |
mitochondrion |
Comments for localization |
mitochondrion? |
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |