Gene name |
SPBC18E5.02c |
Gene ID |
35/D03 |
Gene synonyms/obsolete |
SPBC29A3.20c |
Gene product |
putative serine
palmitoyltransferase subunit |
Entry clone |
Cloned |
ORF length (unspliced) |
1697 |
ORF length (spliced) |
1530 |
Entry clone length |
1697 |
No. of intron |
3 |
Sequence status |
Finished |
Sequence results |
579T:C |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPBC18E5.02.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAGTTACTCATATCCCTT |
Rev primer name |
SPBC18E5.02.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAAACTTGATTAATAACCTTA |
Amino acid length |
509 |
Molecular weight |
57.4 |
Isoelectric point (calc.) |
6.5 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LRTFIEFLLL/LTDVEKLEL/LLEEIVELCI |
Localization (YFP) |
ER |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |