| Gene name |
SPAC22H10.10 |
| Gene ID |
35/D11 |
| Gene synonyms/obsolete |
sto1; alp21 |
| Gene product |
LRR domain; involved
in microtubule assembly (required); tubulin-specific chaperone
and formation of alpha-beta-tubulin heterodimer;
essential |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1705 |
| ORF length (spliced) |
1536 |
| Entry clone length |
1705 |
| No. of intron |
4 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC22H10.10.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCATATATCCACTGGAAT |
| Rev primer name |
SPAC22H10.10.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAGACGTTTCCACTTTCCTTT |
| Amino acid length |
511 |
| Molecular weight |
58.5 |
| Isoelectric point (calc.) |
6.7 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LPEVIDLDL/LFHEFFPILKL/LPSLRNLTL/LQFLQTLSL/LHKLTFLDI/LENLKHLRI |
| Localization (YFP) |
nucleus>cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |