Gene name |
SPAC1B3.16c |
Gene ID |
35/E01 |
Gene synonyms/obsolete |
|
Gene product |
vitamin H transporter;
MFS transporter; biotin transporter; plasma membrane; tandem
duplication; similar to Sp SPAC1B3.16c; functionally
complements Sc VHT1; allantoate permease family |
Entry clone |
Cloned |
ORF length (unspliced) |
1707 |
ORF length (spliced) |
|
Entry clone length |
1707 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC1B3.16.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGCTTCAGAATGGCCTGA |
Rev primer name |
SPAC1B3.16.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAAGCGTCAGCCTGTTGGGAA |
Amino acid length |
568 |
Molecular weight |
62.8 |
Isoelectric point (calc.) |
8 |
Signal SEQ |
|
No. of transmembrane domain |
12 |
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LRRLQKLRL/LRIIPCLWI |
Localization (YFP) |
Golgi; periphery |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |