Gene name |
SPAC31G5.10 |
Gene ID |
35/E09 |
Gene synonyms/obsolete |
|
Gene product |
Myb family;
DNA-binding protein; enhanced transcript after nitrogen
starvation; transcription factor for RNA polymerase II |
Entry clone |
Cloned |
ORF length (unspliced) |
1710 |
ORF length (spliced) |
|
Entry clone length |
1710 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
235A:G / 1054A:G /
1175A:G |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC31G5.10.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGATGTTAGCTATCGACAT |
Rev primer name |
SPAC31G5.10.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATACGTCAGGAGGAAGACTT |
Amino acid length |
569 |
Molecular weight |
65.2 |
Isoelectric point (calc.) |
9.2 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
476/474/475 |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
nucleolus>>nucleus |
Comments for localization |
|
Effect of LMB on protein
localization |
changed to:
intranuclear microtubule bundle (nucleus) |
Microscope used for
observation |
DeltaVision |