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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC31G5.10
Gene ID 35/E09
Gene synonyms/obsolete
Gene product Myb family; DNA-binding protein; enhanced transcript after nitrogen starvation; transcription factor for RNA polymerase II
Entry clone Cloned
ORF length (unspliced) 1710
ORF length (spliced)
Entry clone length 1710
No. of intron 0
Sequence status Finished
Sequence results 235A:G / 1054A:G / 1175A:G
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC31G5.10.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGATGTTAGCTATCGACAT
Rev primer name SPAC31G5.10.Rv
Rev primer SEQ AGAAAGCTGGGTATACGTCAGGAGGAAGACTT
Amino acid length 569
Molecular weight 65.2
Isoelectric point (calc.) 9.2
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) 476/474/475
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleolus>>nucleus
Comments for localization
Effect of LMB on protein localization changed to: intranuclear microtubule bundle (nucleus)
Microscope used for observation DeltaVision

Image information
YFP 2 images) See all images
LMB 4 images) See all images

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