| Gene name |
SPCC126.07c |
| Gene ID |
35/E12 |
| Gene synonyms/obsolete |
|
| Gene product |
zinc finger protein;
zf-PHD finger; zf-C3HC4 type (RING finger); ubiquitin ligase
(E3); involved in transcriptional regulation |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1716 |
| ORF length (spliced) |
|
| Entry clone length |
1716 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
314C:T / 632C:T |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPCC126.07.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAACGCTGAAGAAGATCC |
| Rev primer name |
SPCC126.07.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAGTCATTTGAAAGAGACTGT |
| Amino acid length |
571 |
| Molecular weight |
64.4 |
| Isoelectric point (calc.) |
6.6 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
nucleus>>cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |