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Schizosaccharomyces pombe Postgenome Database

Gene name SPCC4G3.05c
Gene ID 35/F02
Gene synonyms/obsolete mus81
Gene product endodeoxyribonuclease RUS activity; Holliday junction resolvase; involved in DNA repair, nucleotide-excision repair, and nucleotide-excision repair, DNA damage recognition; interacts physically with Cds1p; XP-F family?; involved in meiotic recombination (required) (late step) and the processing of stalled and collapsed replication forks
Entry clone Cloned
ORF length (unspliced) 1719
ORF length (spliced)
Entry clone length 1719
No. of intron 0
Sequence status Finished
Sequence results 1513A:G
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPCC4G3.05.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGAAAAGTTGCCCCATCAC
Rev primer name SPCC4G3.05.Rv
Rev primer SEQ AGAAAGCTGGGTAAGATTCTGGAAAGAAAACG
Amino acid length 572
Molecular weight 64.5
Isoelectric point (calc.) 7.1
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LAVIPDLSI
Localization (YFP) nuclear dots; nucleus; mitochondrion
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 3 images) See all images

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