| Gene name |
SPAC144.03 |
| Gene ID |
35/F09 |
| Gene synonyms/obsolete |
ade2; min10;
min3 |
| Gene product |
adenylosuccinate
synthetase; involved in cadmium resistance (required);
involved in purine biosynthesis |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1727 |
| ORF length (spliced) |
1305 |
| Entry clone length |
1727 |
| No. of intron |
2 |
| Sequence status |
Finished |
| Sequence results |
1453T:C |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC144.03.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGCTTCAGTGCGAGAAAC |
| Rev primer name |
SPAC144.03.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTACTCCTTAACCAACATCTCA |
| Amino acid length |
434 |
| Molecular weight |
47.8 |
| Isoelectric point (calc.) |
6.4 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
cytosol=nucleus |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |