Gene name |
SPAC29A4.20 |
Gene ID |
35/G01 |
Gene synonyms/obsolete |
|
Gene product |
RNA polymerase II
holoenzyme component; histone acetyltransferase |
Entry clone |
Cloned |
ORF length (unspliced) |
1730 |
ORF length (spliced) |
1635 |
Entry clone length |
1730 |
No. of intron |
1 |
Sequence status |
Finished |
Sequence results |
1047T:C |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC29A4.20.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCGACTTCCAGTTTGGC |
Rev primer name |
SPAC29A4.20.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAAAGCCATTTAGACATGTAA |
Amino acid length |
544 |
Molecular weight |
61.8 |
Isoelectric point (calc.) |
8.5 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LVDLIARILAL |
Localization (YFP) |
cytosol; cytoplasmic
dots |
Comments for localization |
bright large dots by
over expression |
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |