Gene name |
SPBC3E7.05c |
Gene ID |
35/G02 |
Gene synonyms/obsolete |
|
Gene product |
hypothetical
protein |
Entry clone |
Cloned |
ORF length (unspliced) |
1730 |
ORF length (spliced) |
1653 |
Entry clone length |
1730 |
No. of intron |
2 |
Sequence status |
Finished |
Sequence results |
458T:C |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPBC3E7.05.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAATCGACAGCTTAGAAC |
Rev primer name |
SPBC3E7.05.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATGCTTGTTGGGCGTCTTCA |
Amino acid length |
550 |
Molecular weight |
63.2 |
Isoelectric point (calc.) |
7.4 |
Signal SEQ |
|
No. of transmembrane domain |
1 |
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LDGSVRALLSL |
Localization (YFP) |
mitochondrion |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |