| Gene name |
SPBC14F5.13c |
| Gene ID |
35/G05 |
| Gene synonyms/obsolete |
|
| Gene product |
vacuolar alkaline
phosphatase; involved in dephosphorylation of
phosphopeptides |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1733 |
| ORF length (spliced) |
1599 |
| Entry clone length |
1733 |
| No. of intron |
1 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC14F5.13.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGCATCTGAGCGCGATCC |
| Rev primer name |
SPBC14F5.13.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAAACAAATCAGCACCGAAA |
| Amino acid length |
532 |
| Molecular weight |
58.6 |
| Isoelectric point (calc.) |
5.2 |
| Signal SEQ |
Predicted
(N-terminus) |
| No. of transmembrane domain |
1 |
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
periphery;
vacuole |
| Comments for localization |
weak signal of
periphery |
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |