Gene name |
SPAC13C5.03 |
Gene ID |
35/G11 |
Gene synonyms/obsolete |
tht1 |
Gene product |
nuclear fusion
protein; required for the fusion of nuclear; envelopes during
karyogamy |
Entry clone |
Cloned (also cloned in
2004 trial) |
ORF length (unspliced) |
1736 |
ORF length (spliced) |
1632 |
Entry clone length |
1736 |
No. of intron |
2 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC13C5.03.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAAATTTCATCCGACGCG |
Rev primer name |
SPAC13C5.03.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAATCCCACCATGGAGATTGC |
Amino acid length |
543 |
Molecular weight |
62.9 |
Isoelectric point (calc.) |
6.3 |
Signal SEQ |
|
No. of transmembrane domain |
3 |
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LSSLFFKVLKI |
Localization (YFP) |
cytoplasmic dots; ER?;
Golgi? |
Comments for localization |
ER-Golgi? |
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |