| Gene name |
SPBC17D1.06 |
| Gene ID |
35/H02 |
| Gene synonyms/obsolete |
|
| Gene product |
DEAD/DEAH box
helicase; ATP-dependent; RNA helicase |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1737 |
| ORF length (spliced) |
|
| Entry clone length |
1737 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
125T:C / 1691T:C |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPCC17D1.06.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCAATTTTGTTTTAGGAT |
| Rev primer name |
SPCC17D1.06.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAGTCAAAAACAATCTTAGTA |
| Amino acid length |
578 |
| Molecular weight |
63.9 |
| Isoelectric point (calc.) |
9.9 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
95/68/69 |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
nucleolus |
| Comments for localization |
|
| Effect of LMB on protein
localization |
changed to:
nucleolus>>nucleus |
| Microscope used for
observation |
DeltaVision |