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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC1683.07
Gene ID 35/H07
Gene synonyms/obsolete
Gene product alpha-glucosidase; alpha-amylase
Entry clone Cloned
ORF length (unspliced) 1740
ORF length (spliced)
Entry clone length 1740
No. of intron 0
Sequence status Finished
Sequence results 746A:G
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBC1683.07.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGAAAGTTGTGCCAAGCGA
Rev primer name SPBC1683.07.Rv
Rev primer SEQ AGAAAGCTGGGTAGAGCTTTAACAAGACTGCT
Amino acid length 579
Molecular weight 67.7
Isoelectric point (calc.) 4.7
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytosol=nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 1 images) See all images

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