Gene name |
SPAC12G12.04 |
Gene ID |
36/A03 |
Gene synonyms/obsolete |
mcp60; hsp60 |
Gene product |
chaperone; involved in
succinate; dehydrogenase assembly; heat shock protein 60;
essential |
Entry clone |
Cloned |
ORF length (unspliced) |
1749 |
ORF length (spliced) |
|
Entry clone length |
1749 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
1242A:G |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC12G12.04.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGTGAGCTTCCTTTCTTC |
Rev primer name |
SPAC12G12.04.Rv |
Rev primer SEQ |
AGAAAGCTGGGTACATCATACCGGGCATTCCA |
Amino acid length |
582 |
Molecular weight |
62.1 |
Isoelectric point (calc.) |
5.7 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LSSSVSRLPL |
Localization (YFP) |
mitochondrion |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Leica |