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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC959.05c
Gene ID 36/A05
Gene synonyms/obsolete
Gene product glycoprotein; thioredoxin related glycoprotein; thioredoxin domain; similar to Sp SPBC3D6.13C and SPCC1840.08C (paralogs)
Entry clone Cloned
ORF length (unspliced) 1750
ORF length (spliced) 1695
Entry clone length 1750
No. of intron 1
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC959.05.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGAGTTTCCTGACATACG
Rev primer name SPAC959.05.Rv
Rev primer SEQ AGAAAGCTGGGTAGTCTGATTTGGCAATTACA
Amino acid length 564
Molecular weight 65.6
Isoelectric point (calc.) 8.6
Signal SEQ
No. of transmembrane domain 1
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LAGVAPLYI/LNRKLTVLTL/LLIALLILSI
Localization (YFP) ER
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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