| Gene name |
SPBC31F10.04c |
| Gene ID |
36/A12 |
| Gene synonyms/obsolete |
srb4 |
| Gene product |
RNA polymerase II
holoenzyme component; mediator complex subunit;
essential |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1754 |
| ORF length (spliced) |
1638 |
| Entry clone length |
1754 |
| No. of intron |
2 |
| Sequence status |
Finished |
| Sequence results |
1340T:C |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC31F10.04.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGCTGAAGAAGCAAACAA |
| Rev primer name |
SPBC31F10.04.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATTCCGTTTTTTTTGACCGT |
| Amino acid length |
545 |
| Molecular weight |
62.4 |
| Isoelectric point (calc.) |
5 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LALDMTSLLL/LGGISLGLGL/LNAIAHILFL |
| Localization (YFP) |
cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Leica |