| Gene name |
SPAC1F5.09c |
| Gene ID |
36/B11 |
| Gene synonyms/obsolete |
shk2; pak2 |
| Gene product |
serine/threonine
protein kinase; pleckstrin homology domain; p21 activated
kinase (PAK); effector for Cdc42p; non-essential; activator of
Mkh1p-Pek1p-Spm1p cascade; Cdc42p/Rac interactive binding
(CRIB) motif |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1770 |
| ORF length (spliced) |
|
| Entry clone length |
1770 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC1F5.09.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCTTTTAAGTGTAAGAGG |
| Rev primer name |
SPAC1F5.09.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAATTAATATGGGTATTCGCT |
| Amino acid length |
589 |
| Molecular weight |
66.7 |
| Isoelectric point (calc.) |
9.7 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LKSFLNSCLTI |
| Localization (YFP) |
mitochondrion |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Leica |