Gene name |
SPBC17G9.03c |
Gene ID |
36/C05 |
Gene synonyms/obsolete |
|
Gene product |
lysine-tRNA ligase;
involved in lysyl-tRNA aminoacylation; lysine-tRNA ligase
activity |
Entry clone |
Cloned in 2006
trial |
ORF length (unspliced) |
1776 |
ORF length (spliced) |
|
Entry clone length |
1776 |
No. of intron |
0 |
Sequence status |
Partially
sequenced |
Sequence results |
100% match in both
ends |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPBC17G9.03c.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAGTGAGGAACAGGTGAA |
Rev primer name |
SPBC17G9.03c.Rv |
Rev primer SEQ |
AGAAAGCTGGGTACTCTTTAACGACAGTAGGT |
Amino acid length |
591 |
Molecular weight |
67.4 |
Isoelectric point (calc.) |
6.2 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LYMRIAPELYL |
Localization (YFP) |
no expression clone
|
Comments for localization |
|
Effect of LMB on protein
localization |
not determined |
Microscope used for
observation |
|