Chemical Genetics Laboratory
PREVIOUS  NEXT
Schizosaccharomyces pombe Postgenome Database

Gene name SPCC645.14c
Gene ID 36/C06
Gene synonyms/obsolete sti1
Gene product TPR repeat protein; activator of Hsp70 and Hsp90 chaperones
Entry clone Cloned
ORF length (unspliced) 1776
ORF length (spliced)
Entry clone length 1776
No. of intron 0
Sequence status Finished
Sequence results 323A:T / 434A:G ; 1595A:G
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPCC645.14.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGCAGAAGAATTGAAAGC
Rev primer name SPCC645.14.Rv
Rev primer SEQ AGAAAGCTGGGTAGCCCAGGCGAATAACACCG
Amino acid length 591
Molecular weight 65.5
Isoelectric point (calc.) 5.1
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytosol=nucleus; cytoplasmic dots
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica, DeltaVision

Image information
YFP 3 images) See all images

For plasmid request Click!
Copyright (c) RIKEN (The Institute of Physical and Chemical Research), Japan. All rights reserved.