Gene name |
SPBC651.09c |
Gene ID |
36/C08 |
Gene synonyms/obsolete |
|
Gene product |
RNA polymerase II
associated Paf1 complex; involved in TATA site selection;
involved in regulation of transcription elongation |
Entry clone |
Cloned |
ORF length (unspliced) |
1780 |
ORF length (spliced) |
1683 |
Entry clone length |
1780 |
No. of intron |
2 |
Sequence status |
Finished |
Sequence results |
145A:G / 1480A:G |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPBC651.09.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGCCGACTTTCAGGATGA |
Rev primer name |
SPBC651.09.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAAATGTTGATATCAATGCCA |
Amino acid length |
560 |
Molecular weight |
63.6 |
Isoelectric point (calc.) |
5.2 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
nucleus |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Leica |