| Gene name |
SPBC2F12.12c |
| Gene ID |
36/C12 |
| Gene synonyms/obsolete |
|
| Gene product |
hypothetical protein;
interacts with ankyrin repeat containing protein by similarity
to D.melanogaster cactin; no apparent Sc ortholog;
non-essential |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1784 |
| ORF length (spliced) |
1554 |
| Entry clone length |
1784 |
| No. of intron |
4 |
| Sequence status |
Finished |
| Sequence results |
541A:G / 1128T:A |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC2F12.12.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGCATTTCGTGATTCAAC |
| Rev primer name |
SPBC2F12.12.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTACCTTCTATGATGAAGCTTT |
| Amino acid length |
517 |
| Molecular weight |
60.8 |
| Isoelectric point (calc.) |
9.5 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LKKTFNEELQI |
| Localization (YFP) |
nucleus;
mitochondrion |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Confocal |