Gene name |
SPBC19F5.03 |
Gene ID |
36/D10 |
Gene synonyms/obsolete |
|
Gene product |
inositol
polyphosphoinositide phosphatase; 2 predicted transmembrane
helices; ER/golgi ATP/ADP exchanger; involved in transport of
ATP into ER which plays a role in Golgi function and actin
cytoskeletal organization |
Entry clone |
Cloned |
ORF length (unspliced) |
1797 |
ORF length (spliced) |
|
Entry clone length |
1797 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
495T:C / 568A:G /
1531T:C |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPBC19F5.03.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGTTCAGTTTGAAGCAAA |
Rev primer name |
SPBC19F5.03.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAAACTTTATGCTTCGAAGCG |
Amino acid length |
598 |
Molecular weight |
68.7 |
Isoelectric point (calc.) |
8.9 |
Signal SEQ |
|
No. of transmembrane domain |
2 |
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LLILTFLGI |
Localization (YFP) |
ER |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Leica |