Gene name |
SPBC1677.03c |
Gene ID |
36/E02 |
Gene synonyms/obsolete |
|
Gene product |
putative threonine
dehydratase precursor; threonine ammonia-lyase activity |
Entry clone |
Cloned |
ORF length (unspliced) |
1803 |
ORF length (spliced) |
|
Entry clone length |
1803 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
626A:G / 723T:C /
1254T:C / 1478A:G |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPBC1677.03.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGACTGGAACGAGTTTTTA |
Rev primer name |
SPBC1677.03.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATTTACGAAGATAGCGCAAG |
Amino acid length |
600 |
Molecular weight |
66.5 |
Isoelectric point (calc.) |
8.6 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
cytoplasmic dots |
Comments for localization |
mitochondrion?;
aggregates by over expression |
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Leica,
DeltaVision |