Gene name |
SPAC1039.02 |
Gene ID |
36/E06 |
Gene synonyms/obsolete |
|
Gene product |
calcineurin-like
phosphoesterase; similar to Sp SPBPB2B2.06C and SPAC17G6.03
(paralogs) |
Entry clone |
Cloned |
ORF length (unspliced) |
1806 |
ORF length (spliced) |
|
Entry clone length |
1806 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
131A:G / 1370T:C /
1748G:A |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC1039.02.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAAGTTATCATCTTTACC |
Rev primer name |
SPAC1039.02.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAATCACAGTAATCACTCCAT |
Amino acid length |
601 |
Molecular weight |
68 |
Isoelectric point (calc.) |
4.7 |
Signal SEQ |
Predicted
(N-terminus) |
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LSSLPSGLGL |
Localization (YFP) |
ER?; cytoplasmic
dots |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Confocal |