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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC328.05
Gene ID 36/E08
Gene synonyms/obsolete
Gene product RNA-binding protein; rrm RNA recognition motif
Entry clone Cloned
ORF length (unspliced) 1808
ORF length (spliced) 1305
Entry clone length 1808
No. of intron 6
Sequence status Finished
Sequence results 449C:T / 1050C:T
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC328.05.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGGTATGATAACTATTGA
Rev primer name SPAC328.05.Rv
Rev primer SEQ AGAAAGCTGGGTAAGAAGGCTCCAAAGAATGT
Amino acid length 434
Molecular weight 48.2
Isoelectric point (calc.) 7.7
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleus>cytosol; nuclear dots
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica, DeltaVision

Image information
YFP 3 images) See all images

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