Gene name |
SPAC3A11.02 |
Gene ID |
36/E10 |
Gene synonyms/obsolete |
scp3 |
Gene product |
zinc finger protein;
zf-CCCH type; spindle poison (isopropyl N-3-chlorophenyl
carbamate) sensitivity protein |
Entry clone |
Cloned in 2006
trial |
ORF length (unspliced) |
1810 |
ORF length (spliced) |
1752 |
Entry clone length |
1810 |
No. of intron |
1 |
Sequence status |
Partially
sequenced |
Sequence results |
100% match in both
ends |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPAC3A11.02.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGACGAAGAAAAATGCCTT |
Rev primer name |
SPAC3A11.02.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAAGCTTCATCCATTTGGAAT |
Amino acid length |
583 |
Molecular weight |
62.8 |
Isoelectric point (calc.) |
8.5 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
cytosol |
Comments for localization |
|
Effect of LMB on protein
localization |
changed to:
nucleus>cytosol (intranuclear microtubule bundle?) |
Microscope used for
observation |
DeltaVision |