Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPCC285.03
Gene ID 36/F06
Gene synonyms/obsolete
Gene product DEAD/DEAH box helicase; ATP-dependent; RNA helicase
Entry clone Cloned
ORF length (unspliced) 1815
ORF length (spliced)
Entry clone length 1815
No. of intron 0
Sequence status Finished
Sequence results 894C:T / 1495C:T
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPCC285.03.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTCTGTTGAAGTAGATAA
Rev primer name SPCC285.03.Rv
Rev primer SEQ AGAAAGCTGGGTAAGTTAAAAAGGACTTTTGT
Amino acid length 604
Molecular weight 68.3
Isoelectric point (calc.) 7.8
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LVKQLRRTLPI
Localization (YFP) nucleus>>cytosol
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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