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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC23A1.12c
Gene ID 36/F08
Gene synonyms/obsolete
Gene product phenylalanine-tRNA ligase (beta subunit); involved in phenylalanyl-tRNA aminoacylation; phenylalanine-tRNA ligase activity
Entry clone Cloned
ORF length (unspliced) 1821
ORF length (spliced) 1770
Entry clone length 1821
No. of intron 1
Sequence status Finished
Sequence results 1577T:C
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC23A1.12.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGCCTACCATCAGCTGTGA
Rev primer name SPAC23A1.12.Rv
Rev primer SEQ AGAAAGCTGGGTAAACCCAAAGTGTCAAATCA
Amino acid length 589
Molecular weight 66.8
Isoelectric point (calc.) 5.1
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytosol=nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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