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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC1687.18c
Gene ID 36/G07
Gene synonyms/obsolete
Gene product hypothetical protein; sequence orphan
Entry clone Cloned
ORF length (unspliced) 1837
ORF length (spliced) 1680
Entry clone length 1837
No. of intron 3
Sequence status Finished
Sequence results 5C:T
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC1687.18.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGACTGGATTGAACGAGAC
Rev primer name SPAC1687.18.Rv
Rev primer SEQ AGAAAGCTGGGTAAACAAAGCTGTATATATCC
Amino acid length 559
Molecular weight 64.7
Isoelectric point (calc.) 7.6
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytosol=nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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