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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC806.08c
Gene ID 36/H04
Gene synonyms/obsolete
Gene product hypothetical protein; sequence orphan
Entry clone Cloned
ORF length (unspliced) 1857
ORF length (spliced)
Entry clone length 1857
No. of intron 0
Sequence status Finished
Sequence results 1261A:G
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC806.08.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGGTATACAAGGTCGGGA
Rev primer name SPAC806.08.Rv
Rev primer SEQ AGAAAGCTGGGTATGGCATTATAAGGAATAAT
Amino acid length 618
Molecular weight 71.5
Isoelectric point (calc.) 5.1
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LHAQILKLFL
Localization (YFP) cytosol=nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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