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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC3H1.04c
Gene ID 37/A01
Gene synonyms/obsolete
Gene product conserved fungal protein
Entry clone Cloned#/Sequence mismatch
ORF length (unspliced) 1907
ORF length (spliced) 1806
Entry clone length 1907
No. of intron 1
Sequence status Finished
Sequence results 1684T:addition
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPAC3H1.04.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGCTCGAATCACGACTTGC
Rev primer name SPAC3H1.04.Rv
Rev primer SEQ AGAAAGCTGGGTATTCGAAATTACTGATTGAA
Amino acid length 601
Molecular weight 69.4
Isoelectric point (calc.) 10.3
Signal SEQ
No. of transmembrane domain 1
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LRRFLHLVL
Localization (YFP) mitochondrion
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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