Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC17G6.03
Gene ID 37/A04
Gene synonyms/obsolete
Gene product calcineurin-like phosphoesterase; similar to Sp SPAC1039.02 and SPBPB2B2.06c (paralogs)
Entry clone Cloned#
ORF length (unspliced) 1908
ORF length (spliced)
Entry clone length 1908
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPAC17G6.03.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGAAACCTGCCCATACATT
Rev primer name SPAC17G6.03.Rv
Rev primer SEQ AGAAAGCTGGGTACTTATATAGGCAATCGTGG
Amino acid length 635
Molecular weight 71.4
Isoelectric point (calc.) 5.5
Signal SEQ Predicted (N-terminus)
No. of transmembrane domain 1
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LDIVLSELRI
Localization (YFP) cytosol; cytoplasmic dots
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 1 images) See all images

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