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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC4G9.04c
Gene ID 37/B04
Gene synonyms/obsolete
Gene product polyadenylation factor
Entry clone Cloned
ORF length (unspliced) 1965
ORF length (spliced) 1917
Entry clone length 1965
No. of intron 1
Sequence status Finished
Sequence results 757A:G / 1759A:G / 1938T:A
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC4G9.04.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGATTTAGTGGAATTGGA
Rev primer name SPAC4G9.04.Rv
Rev primer SEQ AGAAAGCTGGGTACTTGCTTTCTTGTTTAATA
Amino acid length 638
Molecular weight 70.3
Isoelectric point (calc.) 6
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nuclear dots
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Confocal, DeltaVision

Image information
YFP 3 images) See all images

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