Gene name |
SPAC664.11 |
Gene ID |
37/B12 |
Gene synonyms/obsolete |
ssc1; ssp1 |
Gene product |
mitochondrial
chaperonin; heat shock protein 70 family; involved in
mitochondrial protein import; nvolved in heat shock response;
involved in stress response |
Entry clone |
Cloned |
ORF length (unspliced) |
2025 |
ORF length (spliced) |
|
Entry clone length |
2025 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
93A:T |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC664.11.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGATCTCGTCGAGATTCAC |
Rev primer name |
SPAC664.11.Rv |
Rev primer SEQ |
AGAAAGCTGGGTACTTCTTGTCACCCTCAGGA |
Amino acid length |
674 |
Molecular weight |
72.9 |
Isoelectric point (calc.) |
7.1 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LSGHVKDLVL |
Localization (YFP) |
mitochondrion |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |