| Gene name |
SPBC337.17c |
| Gene ID |
37/C04 |
| Gene synonyms/obsolete |
SPBC1734.01c |
| Gene product |
hypothetical protein;
Sc YDR365C is null-letha |
| Entry clone |
Cloned |
| ORF length (unspliced) |
2049 |
| ORF length (spliced) |
|
| Entry clone length |
2049 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
66T:C / 1244T:C /
1572T:C / 1778A:G |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC337.17.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGGCAAAAAGCAGACGAA |
| Rev primer name |
SPBC337.17.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATTTTCCAGAACGTTTAATA |
| Amino acid length |
682 |
| Molecular weight |
77.7 |
| Isoelectric point (calc.) |
4.5 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
635/637 |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
nucleolus>>nucleus |
| Comments for localization |
bright signal |
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Leica |