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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC776.10c
Gene ID 37/E04
Gene synonyms/obsolete
Gene product golgi transport complex (peripheral subunit); involved in intracellular protein transport; involved in secretory pathway; involved in ER to golgi transport
Entry clone Cloned
ORF length (unspliced) 2136
ORF length (spliced) 2028
Entry clone length 2136
No. of intron 2
Sequence status Finished
Sequence results 403A:G
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBC776.10.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGATGTGAAATCCAAGCA
Rev primer name SPBC776.10.Rv
Rev primer SEQ AGAAAGCTGGGTAGTTTACTGATAAATTGTCT
Amino acid length 675
Molecular weight 77.3
Isoelectric point (calc.) 4.5
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LSETIDNLQL
Localization (YFP) nucleus>=cytosol; periphery at site of septum formation
Comments for localization aggregates by over expression
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 2 images) See all images

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