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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC2E1P3.04
Gene ID 37/E06
Gene synonyms/obsolete
Gene product copper amine oxidase; no apparent Sc ortholog
Entry clone Cloned
ORF length (unspliced) 2139
ORF length (spliced)
Entry clone length 2139
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC2E1P3.04.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGCTTATTATCCTCACCT
Rev primer name SPAC2E1P3.04.Rv
Rev primer SEQ AGAAAGCTGGGTAAAAGAAGTGATCACCTTTA
Amino acid length 712
Molecular weight 81.2
Isoelectric point (calc.) 6.5
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytosol
Comments for localization aggregates; cytoplasmic dots by over expression
Effect of LMB on protein localization changed to: cytosol=nucleus
Microscope used for observation Leica

Image information
YFP 4 images) See all images
LMB 4 images) See all images

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