| Gene name |
SPBC18H10.02 |
| Gene ID |
37/F06 |
| Gene synonyms/obsolete |
|
| Gene product |
long-chain-fatty-acid-CoA ligase (putative); fatty
acyl-CoA synthetase (putative); involved in the maintenance of
viability in stationary phase; similar to Sp SPBP4H10.11C
(paralog) |
| Entry clone |
Cloned |
| ORF length (unspliced) |
2157 |
| ORF length (spliced) |
2031 |
| Entry clone length |
2157 |
| No. of intron |
1 |
| Sequence status |
Finished |
| Sequence results |
1897A:G /
2042T:C |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC18H10.02.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAAGGTACAATCTAAGGG |
| Rev primer name |
SPBC18H10.02.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTACTCGGCGTAAGCGGCGTCA |
| Amino acid length |
676 |
| Molecular weight |
75.9 |
| Isoelectric point (calc.) |
8.3 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
ER |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Leica |