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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC9G1.05
Gene ID 37/F11
Gene synonyms/obsolete
Gene product WD repeat protein; actin cortical patch component; involved in stress response
Entry clone Cloned
ORF length (unspliced) 2160
ORF length (spliced) 1788
Entry clone length 2160
No. of intron 6
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC9G1.05.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTCTTCTCAGTTAAAATC
Rev primer name SPAC9G1.05.Rv
Rev primer SEQ AGAAAGCTGGGTAAAAGGTCACTGACCATACT
Amino acid length 595
Molecular weight 65.2
Isoelectric point (calc.) 6.5
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytoplasmic dots at cell tip and site of septum formation; cytosol=nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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