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Schizosaccharomyces pombe Postgenome Database

Gene name SPCC645.13
Gene ID 37/G04
Gene synonyms/obsolete
Gene product zinc finger protein; zf-PHD finger; TFS2M domain; tranSpriptional regulator; involved in transcriptional regulation
Entry clone Cloned
ORF length (unspliced) 2166
ORF length (spliced)
Entry clone length 2166
No. of intron 0
Sequence status Finished
Sequence results 1239A:G
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPCC645.13.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGTAAGAAAAAGCACTAG
Rev primer name SPCC645.13.Rv
Rev primer SEQ AGAAAGCTGGGTATTGTTTGGACGCCTTTTTC
Amino acid length 721
Molecular weight 79.7
Isoelectric point (calc.) 5.1
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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