Chemical Genetics Laboratory
PREVIOUS  NEXT
Schizosaccharomyces pombe Postgenome Database

Gene name SPBC6B1.05c
Gene ID 38/A01
Gene synonyms/obsolete
Gene product involved in autophagy; involved in vacuole targeting; involved in peroxisome degradation
Entry clone Cloned
ORF length (unspliced) 2206
ORF length (spliced) 1950
Entry clone length 2206
No. of intron 6
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBC6B1.05.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTTTGTTGGAAAGGCATT
Rev primer name SPBC6B1.05.Rv
Rev primer SEQ AGAAAGCTGGGTAAAGAATCACACTTTCTTTA
Amino acid length 649
Molecular weight 73.3
Isoelectric point (calc.) 6.1
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleus>cytosol
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

For plasmid request Click!
Copyright (c) RIKEN (The Institute of Physical and Chemical Research), Japan. All rights reserved.