| Gene name |
SPBC17G9.08c |
| Gene ID |
38/A02 |
| Gene synonyms/obsolete |
csx2 |
| Gene product |
ADP-ribosylation
factor (ARF); GTPase activating protein; ArfGap domain;
pleckstrin homology domain; similar to Sp SPAC26A3.10; no
apparent Sc ortholog |
| Entry clone |
Cloned |
| ORF length (unspliced) |
2932 |
| ORF length (spliced) |
2613 |
| Entry clone length |
2932 |
| No. of intron |
5 |
| Sequence status |
Finished |
| Sequence results |
447A:G / 631A:G /
2440A:G / 2906T:G |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC17G9.08.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGACTGGGCATGTCGATCC |
| Rev primer name |
SPBC17G9.08.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAGGAGTTGCTCTATTCATC |
| Amino acid length |
870 |
| Molecular weight |
99 |
| Isoelectric point (calc.) |
6.8 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LSGNVDPLPI/LKEAVLCLAL |
| Localization (YFP) |
cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Leica |