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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC19G7.01c
Gene ID 38/A03
Gene synonyms/obsolete msh2; SPBC24C6.12c
Gene product mismatch-binding protein; involved in DNA repair; involved in mismatch repair; MMR pathway; interacts physically with Msh1p; interacts physically with Msh6; involved in mating-type switching; involved in chromosome organization (meiotic)
Entry clone Cloned
ORF length (unspliced) 3086
ORF length (spliced) 2949
Entry clone length 3086
No. of intron 2
Sequence status Finished
Sequence results 7T:C / 478G:A
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBC19G7.01.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTCTTCTAGAAACGCTTC
Rev primer name SPBC19G7.01.Rv
Rev primer SEQ AGAAAGCTGGGTATGAGGAAACCTCATTTGTG
Amino acid length 982
Molecular weight 109.7
Isoelectric point (calc.) 6.1
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytosol=nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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