| Gene name |
SPAC328.04 |
| Gene ID |
38/A08 |
| Gene synonyms/obsolete |
|
| Gene product |
AAA family ATPase;
CDC48/PAS1/SEC18 family; similar to katanin; putative
microtubule severing protein by similarity |
| Entry clone |
Cloned |
| ORF length (unspliced) |
2226 |
| ORF length (spliced) |
|
| Entry clone length |
2226 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC328.04.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAGCCTTGAAGACTGCTA |
| Rev primer name |
SPAC328.04.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAATGCCCTTGGGATCCGAAC |
| Amino acid length |
741 |
| Molecular weight |
81.7 |
| Isoelectric point (calc.) |
8.2 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LLCMLEQLTI |
| Localization (YFP) |
cytoplasmic dots
|
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Leica |