| Gene name |
SPAC7D4.12c |
| Gene ID |
38/B05 |
| Gene synonyms/obsolete |
|
| Gene product |
hypothetical protein;
Sc YJL108C is regulated by pheromone; similar to Sp
SPAC7D4.12c |
| Entry clone |
Cloned |
| ORF length (unspliced) |
2280 |
| ORF length (spliced) |
|
| Entry clone length |
2280 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
305A:G / 370A:G /
1452A:G |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC7D4.12.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGACGGAAATCGTAGAGT |
| Rev primer name |
SPAC7D4.12.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAAAACTAAATAAGCCAGAT |
| Amino acid length |
759 |
| Molecular weight |
83.3 |
| Isoelectric point (calc.) |
8.4 |
| Signal SEQ |
|
| No. of transmembrane domain |
9 |
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LFVPLFTLCL |
| Localization (YFP) |
ER |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Leica |