Gene name |
SPACUNK4.08 |
Gene ID |
38/D03 |
Gene synonyms/obsolete |
|
Gene product |
dipeptidyl
aminopeptidase; peptidase family S9; predicted N-terminal
signal anchor; prolyl endopeptidase |
Entry clone |
Cloned in 2006
trial |
ORF length (unspliced) |
2382 |
ORF length (spliced) |
|
Entry clone length |
2382 |
No. of intron |
0 |
Sequence status |
Partially
sequenced |
Sequence results |
100% match in both
ends |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPACUNK4.08.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAATGATTTTAGTTTTGA |
Rev primer name |
SPACUNK4.08.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAATCCCATCGATGTTGTCGA |
Amino acid length |
793 |
Molecular weight |
91.3 |
Isoelectric point (calc.) |
4.7 |
Signal SEQ |
Predicted
(N-terminus) |
No. of transmembrane domain |
1 |
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LALIFSLLIL |
Localization (YFP) |
no apparent
signal |
Comments for localization |
|
Effect of LMB on protein
localization |
not determined |
Microscope used for
observation |
Leica |