Gene name |
SPAC19B12.01 |
Gene ID |
38/D07 |
Gene synonyms/obsolete |
SPAC4F10.21 |
Gene product |
hypothetical protein;
TPR repeat protein |
Entry clone |
Cloned |
ORF length (unspliced) |
2454 |
ORF length (spliced) |
|
Entry clone length |
2454 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
1691C:T |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC4F10.21.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGTAGCAATAGAAAATAA |
Rev primer name |
SPAC4F10.21.Rv |
Rev primer SEQ |
AGAAAGCTGGGTACTTCAAATTTTCTAGTTCA |
Amino acid length |
817 |
Molecular weight |
94.2 |
Isoelectric point (calc.) |
5.4 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LAPKLLFLLL |
Localization (YFP) |
cytosol=nucleus |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Leica |