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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC19B12.05c
Gene ID 38/D09
Gene synonyms/obsolete fcp1
Gene product serine phosphatase; TFIIF-interacting C-terminal domain phosphatase; BRCT domain; NLI interacting factor family; metal-dependent phosphotransferase; interacts physically with Rpb4p; CTD phoshphatase; essential
Entry clone Cloned
ORF length (unspliced) 2463
ORF length (spliced) 2172
Entry clone length 2463
No. of intron 5
Sequence status Finished
Sequence results 1467A:G / 1755A:G
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC19B12.05.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTCGAAACGATTGACACC
Rev primer name SPAC19B12.05.Rv
Rev primer SEQ AGAAAGCTGGGTAAGCTGTATCTTTGGACAAT
Amino acid length 723
Molecular weight 81.9
Isoelectric point (calc.) 4.4
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) 659
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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